Research of ethanol plantex tracts antimicrobial properties and synergy with erythromyc in against skinisolates of MLS-resistant staphylococci
Screening of 48 water-ethanolic plants extracts direct antimicrobial activity and synergy with erythromycin against 3 skin isolates of staphylococci with various mechanisms of MLS-resistance (macrolide, lincosamide and streptogramin B): inducible, constitutive and full resistance was performed by agar diffusion method. Strong antimicrobial activity of Betula verrucosa L. and Populus nigra L. buds, Polygonum bistorta L. rhizomes, Punica granatum L. fruit pericarp, Biota orientalis L.needles and fruits extracts against all tested strains was established. Betula verrucosa L. buds and Alnus incana L. fruit extracts showed synergic action with 1/ 4 and 1/64 MIC (minimal inhibitory concentration) of erythromycin against all tested strains of staphylococci. Biologically active substances of medicinal plants effectively restore sensitivity to erythromycin in staphylococci with low-level MLS-resistance by blocking of efflux pumps. High-level MLS-resistance has been modified significantly weaker.