Comparative sesitivity of cell cultures to enteroviruses clinical isolates
In the research there were presented the results of determination of selection of optimal combinations of different cell cultures which can be used for enteroviruses isolation from clinical material. Prototypical vaccine strains of poliovirus I type (strain Lsc2ab) and Coxsackie viruses B6 (strain Hammon), also clinical isolated enteroviruses obtained from patients with dysbiotic disorders are used in research. There were presented the results of research of such types of cell cultures as RD, HEp-2, Vero, HeLa, L20B, L41 (mouse fibroblasts) are studied with comparison. There was identified the optimal combination of cell cultures for monitoring of enterovirus infections spreading with the context of the polio eradication program: cell culture L20B - for polioviruses detection and HEp-2 - for polio and another enteroviruses specieses detection. The retardation of cell culture cytopathic effect after infection with viral clinical isolate was determined. Obtained results should be considered during enteroviruses isolation from patient material and environment.