Comparative evaluation of the effect of amantadine sulphate and memantine on the intensification of neurocy to destructive, apoptotic and proliferativ echanges in the retina of rabbits with a contusion of the visual analyzer
The first 7 days of the experimental eye contusion, caused by exposure to the visual analyzer of carbon dioxide flow under pressure, characterized by the simultaneous intensification of necrotic (neurocytodestructive), apoptotic and proliferative processes in the retina of rabbits. This was evidenced by a significant relative increase in the indices of intact animals NSE activity in average 43,3 fold increase in the percentage of cells with nuclear DNA fragmentation signs (apoptosis), and increase the number of cells in the DNA synthesis phase (phase S - proliferative activity) to the total number of tissue suspension on average 14,66 times, and 4,41 (p<0,05), as well as significant escalation of S100 protein titers on 7 day іn 39,7 times. Amantadine sulphate solution dose of 2.5 mg/kg intravenously (i/v) and pelletized form of memantine 20 mg/kg intragastric (i/g) to have reduced relative to the control group pathology percentage of cells with nuclear DNA fragmentation signs on 51,1 and 25,3%, and those which are in S-phase of the cell cycle respectively on average on 44,0 and 25,3 % (p<0,05). At the same time, the effectiveness of the original drug PK-Merz (amantadine sulfate) was significantly higher for oral adamantane antiapoptotic activity on average 34,5 %, and exceeded its antiproliferative effect by on 25 %. Separate administration of amantadine sulphate or memantine contributed accurate de-escalation in the blood of rabbits NSE activity after 24 hours of the experiment an average of 1,89 and 1,59 times, and the S100 protein titers on 7 day in the eyes contusion 1,74 and 1,53 times, respectively. Both drugs as memantine, and amantadine sulfate, are carriers neurocytodestructive activity that is associated with the implementation of the background to their administration in a contusion cytoprotective eyes anitapoptotic and antiproliferative action. By its efficiency in terms of the model eye contusion, amantadine sulfate conditionally effective dose of 2,5 mg/kg i/v in significantly superior memantine 20 mg/kg i/g.