Biofilmproducing properties of clinical strains of grampositive microorganisms

  • O.A. Nazarchuk
  • M.O. Faustova
Keywords: biofilms, infectious-inflammatory complications, microorganisms.


In more than 90% of cases the implant osseointegration in the alveolar bone is reported to be successful, but implant placement may also be accompanied by some complications that can occur during the procedure of dental implant placement as well as in the postoperative period. The article presents the results of the study of bio-film-forming properties of clinical strains of gram-positive microorganisms, pathogens of infectious-inflammatory complications of odontoplasty. Biofilm-forming properties were investigated on 145 clinical strains of conditionally pathogenic microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus warneri, Streptococcus sunguinis та Kocuria kristinae), isolated from patients with infectious odonto-implant related complications, using the spectrophotometric method (MtP-test «microtiter plate test», by G.D. Christensen). Clinical strains of S. aureus, S. epidermidis and S. sanguinis have been established to have high biofilm-forming properties, and K. kristinae – obtained medium ones. S. warneri had the lowest biofilm formation.


1. Golub, A. V. (2012). Bakterialnye bioplenki – novaya cel terapii. Klinicheskaya mikrobiologiya i antimikrobnaya himioterapiya, 14 (1), 23-29. [in Russian].

2. Faustova, M. O., Nazarchuk, O. A., & Ananieva, M. M. (2017). Protystreptokokova aktyvnist antybiotykiv i antyseptykiv. Aktualni problemy suchasnoi medytsyny: Visnyk ukrainskoi medychnoi stomatolohichnoi akademii, 2 (58), 58-60. [in Ukrainian].

3. Christensen, G. D., Simpson, W. A., Younger, J. J., Baddour, L. M., Barrett, F. F., Melton, D. M., & Beachey E. H. (1985), Adherence of Coagulase-Negative Staphylococci to Plastic Tissue Culture Plates: a Quantitative Model for the Adherence of Staphylococci to Medical Devices. Journal of clinical microbiology, 22(6), 996–1006.

4. Kandi, V., Palange, P., Vaish, R., Bhatti, A. B., Kale, V., Kandi, M. R., & Bhoomagir, M. R. (2016). Emerging Bacterial Infection: Identification and Clinical Significance of Kocuria Species. Cureus, 8(8): e731. doi: 10.7759/cureus.731.

5. Lee, A., & Wang, H. L. (2010). Biofilm related to dental implants. Implant Dentistry, 19(5), 387–393. doi: 10.1097/ID.0b013e3181effa53.

6. Pace, J. L., Rupp, M., & Finch, R. G. (Eds.). (2006). Biofilms, infection, and antimicrobial therapy. Boca Raton: Taylor & Francis Group.

7. Phillips, P. L., Wolcott, R. D., Fletcher, J., & Schultz, G. S. (2010). Biofilms Made Easy. Wounds International, 1(3). Retreived from:

8. Shahabouee, M., Rismanchian, M., Yaghini, J., Babashahi, A., Badrian, H., & Goroohi, H. (2012). Microflora around teeth and dental implants. Dental Research Journal, 9(2), 215–220. Retreived from:

9. Vasanthi, R., Karthikeyan, D., & Jeya, M., (2014). Study of biofilm production and antimicrobial resistance pattern of the bacterial isolates from invasive devices. International Journal of Research in Health Sciences, 2(1), 274–280.
How to Cite
Nazarchuk, O., & Faustova, M. (2017). Biofilmproducing properties of clinical strains of grampositive microorganisms. Biomedical and Biosocial Anthropology, (29), 7-10. Retrieved from

Most read articles by the same author(s)