Influence of the intestinal microbiota to the reproduction of enteroviruses in the experiment


  • V.V. Bobyr
  • V.A. Ponyatovsky
  • O.M. Dyuzhikova
  • O.A. Nazarchuk
  • V.B. Nastenko
  • V.P. Shirobokov
Keywords: antibiotics, dysbiosis, enteroviruses, infection, intestinal microflora.

Abstract

The results of the study of the influence of bacterial microflora of the intestine on the duration of the preservation of enterovirus infectivity in in vivo and in vitro conditions are presented in this work. According to the results of the study, the ability of enteroviruses to maintain infectious activity in fecal masses of animals with preserved microflora and in conditions of antibiotic induced dysbiosis has been shown. The connection between the growth of virus titres in fecal masses of animals and the presence of microorganisms has been established. Increased viability of the polio virus of type 1 (Lsc2ab strain) has been shown during storage in the presence of bacteria. The presence of Bacillus subtilis and Lactobacillus plantarum has been established to the best contributing in maintaining the infectivity of viruses to 60 days. The scientific hypothesis about the possible antiviral effect of antibiotics on the background of formation of dysbiotic states has been formulated.

References

1. Grohmann, G. R., Glass, I., Gold, J., James, M., Edwards, P., Gold, J…. Monroe, S. S. (1991). Outbreak of human calicivirus gastroenteritis in a day-care center in Sydney, Australia. Journal of Clinical Microbiology, 29, 544–550.

2. Hanahan, D., & Weinberg R. A. (2011). Hallmarks of cancer: the next generation. Cell, 144(5), 646–674.

3. Handley, S. A., Thackray, L. B., Zhao, G., & Bushman, F. D. (2012). Pathogenic simian immunodeficiency virus infection is associated with expansion of the enteric virome. Cell, 151. 253–266.

4. Karst, S. M., Wobus, C. E., Lay, M., Davidson, J., & Virgin, H. W. (2003). STAT1-dependentinnate immunity to a Norwalk-like virus. Science, 299, 1575–1578.

5. Kernbauer, E., Ding, Y., & Cadwell, K. (2014). An enteric virus can replace the beneficial function of commensal bacteria. Nature, 516, 94–98.

6. Liste, M. B., Natera, l., Suare, J. A., Pujol, F. H., Liprandi, F., & Ludert, J. E. (2000). Enteric Virus Infections and Diarrhea in Healthy and Human Immunodeficiency Virus-Infected Children. Journal of Clinical Microbiology, 38(8), 2873–2877.

7. Michael, C. (2014, January 22). Purdy Viruses may play unexpected role in inflammatory bowel diseases. Retrieved from http: //news.wustl.edu/news/Pages/27891.aspx.

8. Minot, S., Sinha, R., Chen, J., Li, H., Keilbaugh, S. A., Wu, D. G… Bushman, F. D. (2011). The human gut virome: inter-individual variation and dynamic response to diet. Genome Research, 21, 1616–1625.

9. Modi, S. R., Lee, H. H., Spina C. S., & Collins J. J. (2013). Antibiotic treatment expands the resistance reservoir and ecological network of the phage metagenome. Nature, 499, 219–222.

10. Ninomiya, M., Takahashi, M., Nishizawa, T., Shimosegawa T., & Okamoto H. J. (2008). Development of PCR assays with nested primers specific for differential detection of three human anellovirusesandearly acquisition of dual or triple infection during infancy. Journal of Clinical Microbiology, 46, 507–514.

11. Norman, J. M., Handley, S. A., & Virgin, H. W. (2014). Kingdom-agnostic metagenomics and the importance of complete characterization of enteric microbial communities. Gastroenterology, 146, 1459–1469.

12. Ott, C., L. Duret, L., Chemin, I., Trépo, C., Mandrand, B., & Komurian-Pradel F. (2000). Use of a TT virus ORF1 recombinant protein to detect anti-TT virus antibodies in human sera. Journal of General Virology, 81, 2949–2958.

13. Papaventsis, D., Markoulatos, P., Mangafas, N., Marios, L., Lazanas, M., & Levidiotou-Stefanou, S. (2004). Enteroviral Infection in Greek AIDS Patients. Moltcular Diagnosis, 8(1), 11–16.

14. Sharon, K. K.,. Gavin, T. B., & Chris, A. E. (2011). Intestinal microbiota promote enteric virus replication and systemic pathogenesis. Science, 334(6053), 249–252.

15. Shirobokov, V. P., Bobyr, V. V., Doan, S. I., Shcherbinskaya, A. M. & Ponyatovski V. A. (2012). Enteric viruses have spread the word HIV-infected. Preventive medicine, 1(17), 22–25.

16. Virgin, H. W. (2014). The virome in mammalian physiology and disease. Cell, 157, 142–150.

17. Wobus, C. E., Karst, S. M., Thackray, L. B., Chang, K., Sosnovtsev, S. V., Belliot, G….Virgin, H.W. (2004). Replication of norovirus in cell culture reveals a tropism for dendritic cells and macrophages. PLoS Biology, 2(12), 2076–2084.

18. Xi, Z., Ramirez, J. L., & Dimopoulos, G. (2008). The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathogens, 4(2), 12.

19. Бобир, В. В., Понятовський, В. А., Дюжикова, О. М., & Широбоков, В. П. (2015). Способи моделювання дисбіотичних порушень на лабораторних тваринах Biomedical and Biosocial Anthropology, 24, 230–233.

20. ВООЗ. (2005). Руководство по вирусологическим исследованиям полиомиелита. Женева.

21. Про захист тварин від жорстокого поводження. № 3447-15 § розд. 27 ст. 230 (2006).
Published
2017-10-27
How to Cite
Bobyr, V., Ponyatovsky, V., Dyuzhikova, O., Nazarchuk, O., Nastenko, V., & Shirobokov, V. (2017). Influence of the intestinal microbiota to the reproduction of enteroviruses in the experiment. Biomedical and Biosocial Anthropology, (29), 10-15. Retrieved from https://bba-journal.com/index.php/journal/article/view/276