Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0.9% NaCl solution


  • A.O. Gavryluk
  • I.V. Gunas
  • G.M. Galunko
  • I.L. Chereshniuk
  • D.A. Lysenko
Keywords: skin burn, cell cycle, small intestine, DNA cytometry.

Abstract

The dynamics of changes in the parameters of synthesis and apoptosis (S-phase and SUB-G0G1 interval), as well as other parameters of the cell cycle of cells of the mucous membrane of the small intestine with burn injuries of the skin after 14, 23 and 30 days on the background of the previous injection the first seven days of 0.9% solution of NaCl was investigated using the method of DNA cytometry. 14 days after the skin burn injury in the background of using 0.9% NaCl solution, lower decreases in S-phase and proliferation (p<0,05) were observed than in the non-burning skin group. After 21 days, the level of the S-phase is practically restored, and the SUB-G0G1 interval exceeds the values (p<0,05) obtained in the group without skin burns. After 30 days, the G2+M (p<0,05) and the proliferation index increased, while the proliferation and G0G1 phase decreased (p<0,05). Thus, 30 days after the thermal trauma of the skin there is insufficient recovery of proliferative activity against the background of application of 0.9% solution of NaCl.

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Published
2017-10-27
How to Cite
Gavryluk, A., Gunas, I., Galunko, G., Chereshniuk, I., & Lysenko, D. (2017). Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0.9% NaCl solution. Biomedical and Biosocial Anthropology, (29), 104-108. Retrieved from https://bba-journal.com/index.php/journal/article/view/295