Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0.9% NaCl solution

  • A.O. Gavryluk
  • I.V. Gunas
  • G.M. Galunko
  • I.L. Chereshniuk
  • D.A. Lysenko
Keywords: skin burn, cell cycle, small intestine, DNA cytometry.


The dynamics of changes in the parameters of synthesis and apoptosis (S-phase and SUB-G0G1 interval), as well as other parameters of the cell cycle of cells of the mucous membrane of the small intestine with burn injuries of the skin after 14, 23 and 30 days on the background of the previous injection the first seven days of 0.9% solution of NaCl was investigated using the method of DNA cytometry. 14 days after the skin burn injury in the background of using 0.9% NaCl solution, lower decreases in S-phase and proliferation (p<0,05) were observed than in the non-burning skin group. After 21 days, the level of the S-phase is practically restored, and the SUB-G0G1 interval exceeds the values (p<0,05) obtained in the group without skin burns. After 30 days, the G2+M (p<0,05) and the proliferation index increased, while the proliferation and G0G1 phase decreased (p<0,05). Thus, 30 days after the thermal trauma of the skin there is insufficient recovery of proliferative activity against the background of application of 0.9% solution of NaCl.


1. Halunko H. M. (2017). Histolohichni zminy v tonkii kyshtsi u pizni stadii opikovoi khvoroby. Svit medytsyny ta biolohii, 3 (61), 90-96. [in Ukrainian].
2. Halunko, H. M., & Havryliuk, A. O. (2014). Elektronno-mikroskopichni zminy slyzovoi obolonky tonkoi kyshky shchuriv v pizni stadii opikovoi khvoroby. Svit medytsyny ta biolohii, 4 (47), 97-101. [in Ukrainian].
3. Makarova, O. I., & Chaikovskyi, Yu. B. (2014). Osoblyvosti ultrastrukturnykh zmin v respiratornomu viddili lehen shchuriv u viddalenyi period pislia termichnoi travmy za umov yii korektsii koloidno-hiperosmoliarnym infuziinym rozchynom HAES-LX-5%. Svit medytsyny ta biolohii, 4 (46), 115-120. [in Ukrainian].
4. Stefanov, O. V. (2001). Doklinichni doslidzhennia likarskykh zasobiv. Metodychni rekomendatsii. Kyiv : Avitsena. [in Ukrainian].
5. Fistal, E. Ya., Kozinec, G. P., Samojlenko, G. E., Nosenko, V. M., Fistal, N. N., Soloshenko, V. V. (2005). Kombustiologiya: Uchebnik. Doneck : [b.i.]. [in Russian].
6. Auger, C., Samadi, O., Jeschke, M. G. (2017). The biochemical alterations underlying post-burn hypermetabolism. Biochim Biophys Acta, pii:S0925-4439(17)30068-6.
7. De Jong, P. R., González-Navajas, J. M., Jansen, N. J. G. (2016). The digestive tract as the origin of systemic inflammation. Critical Care, 20, 279.
8. Grimes, L., Doyle, A., Miller, A. L., Pyles, R. B., Olah, G., Szabo, C., … Eaves-Pyles, T. (2016). Intraluminal Flagellin Differentially Contributes to Gut Dysbiosis and Systemic Inflammation following Burn Injury. PLoS ONE, 11 (12), e0166770.
9. Gunas, I., Dovgan, I., Masur, O. (1997). Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München : Der Urban & Fischer Verlag.
10. Husain, K. D., Stromberg, P. E., Woolsey, C. A., Turnbull, I. R., Dunne, W. M., Javadi, P., … Coopersmith, C. M. (2005). Mechanisms of decreased intestinal epithelial proliferation and increased apoptosis in murine acute lung injury. Critical care medicine, 33 (10), 2350-2357.
11. Lee, M. O., (1989). Determination of the surface area of the white rat with its application to the expression of metabolic results. Am. J. Physiol., 24, 1223.
12. Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. J. Trauma, 32, 5, 557-563.
13. Varedi, M., Greeley, G. H. Jr., Herndon, D. N., Englander, E. W. (1999). A thermal injury-induced circulating factor(s) compromises intestinal cell morphology, proliferation, and migration. American Journal of Physiology - Gastrointestinal and Liver Physiology, 277 (1), 175-182.
14. Williams, J. M., Duckworth, C. A., Burkitt, M. D., Watson, A. J., Campbell, B. J., Pritchard, D. M. (2015). Epithelial Cell Shedding and Barrier Function: A Matter of Life and Death at the Small Intestinal Villus Tip. Veterinary Pathology, 52 (3), 445-455.
15. Wolf, S. E., Ikeda, H., Matin, S., Debroy, M. A., Rajaraman, S., Herndon, D. N., Thompson, J. C. (1999). Cutaneous burn increases apoptosis in the gut epithelium of mice. J. Am. Coll. Surg., 188 (1), 10-16.
How to Cite
Gavryluk, A., Gunas, I., Galunko, G., Chereshniuk, I., & Lysenko, D. (2017). Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0.9% NaCl solution. Biomedical and Biosocial Anthropology, (29), 104-108. Retrieved from