Evaluation of the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats
Various pathological conditions can be characterized not only by a decrease or increase in basal levels of hydrogen sulfide in the serum, but also the levels of hydrogen sulfide can modulate the course of the pathological process. The impact of serum hydrogen sulfide on the condition of the intact vaginal wall of rats was evaluated in this study. The aim of the study was to evaluate the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats. The study was performed on 75 female Wistar rats under 1 year of age and weighing 160.0 to 200.0 grams. All animals were divided into 6 groups: control (intact rats); experimental 1 (H2S excess); experimental 2 (H2S deficiency); experimental 3 (intravaginal administration of suppositories with clindamycin); experimental 4 (H2S excess + suppositories with clindamycin); experimental 5 (H2S deficiency + suppositories with clindamycin). The levels of serum hydrogen sulfide were studied, as well as microscopic examination of the structure of the vaginal wall and determination of the levels of TNF-α and IL-1β in tissue homogenate were performed. In experimental groups 3, 4 and 5 all studies were performed in dynamics – 10 minutes, 4, 8 and 24 hours after a single intravaginal administration of clindamycin phosphate. The data were processed using the statistical software package SPSS 20.0 for Windows. Under conditions of both hydrogen sulfide deficiency and excess, no statistically significant changes in TNF-α and IL-1β levels in the vaginal wall of intact rats were observed. Also, no changes in the histological structure of the wall were found. Similar data were demonstrated in experimental groups 3, 4 and 5. This picture is explained by the fact that hydrogen sulfide affects various parts of the inflammatory process, while reducing the production of inflammatory mediators. In intact tissues, in the absence of an inflammatory process, there is no point of application of hydrogen sulfide, and therefore no significant changes are observed. Thus, both excess and deficiency of serum hydrogen sulfide do not affect the condition of the vaginal wall of intact rats.
 Benedetti, F., Curreli, S., Krishnan, S., Davinelli, S., Cocchi, F., Scapagnini, G., … & Zella, D. (2017). Anti-inflammatory effects of H2S during acute bacterial infection: a review. Journal of translational medicine, 15(1), 100. doi: 10.1186/s12967-017-1206-8
 Bhatia, M. (2012). Role of hydrogen sulfide in the pathology of inflammation. Scientifica, 2012, 159680. doi: 10.6064/2012/159680
 Bhatia, M. (2015). H2S and inflammation: an overview. Chemistry, Biochemistry and Pharmacology of Hydrogen Sulfide, 230, 165-180. doi: 10.1007/978-3-319-18144-8_8
 Catalone, B. J., Kish-Catalone, T. M., Budgeon, L. R., Neely, E. B., Ferguson, M., Krebs, F. C., … & Wigdahl, B. (2004). Mouse model of cervicovaginal toxicity and inflammation for preclinical evaluation of topical vaginal microbicides. Antimicrobial agents and chemotherapy, 48(5), 1837-1847. doi: 10.1128/aac.48.5.1837-1847.2004
 Cirino, G., Vellecco, V., & Bucci, M. (2017). Nitric oxide and hydrogen sulfide: the gasotransmitter paradigm of the vascular system. British journal of pharmacology, 174(22), 4021-4031. doi: 10.1111/bph.13815
 Eastment, M. C., & McClelland, R. S. (2018). Vaginal microbiota and susceptibility to HIV. AIDS (London, England), 32(6), 687-698. doi: 10.1097/QAD.0000000000001768
 Fischbach, F., Petersen, E. E., Weissenbacher, E. R., Martius, J., Hosmann, J., & Mayer, H. (1993). Efficacy of clindamycin vaginal cream versus oral metronidazole in the treatment of bacterial vaginosis. Obstetrics and gynecology, 82(3), 405-410.
 Fu, X. Y., Chen, H. H., Zhang, N., Ding, M. X., Qiu, Y. E., Pan, X. M., … & Wang, W. Q. (2018). Effects of chronic unpredictable mild stress on ovarian reserve in female rats: Feasibility analysis of a rat model of premature ovarian failure. Molecular medicine reports, 18(1), 532-540. doi: 10.3892/mmr.2018.8989
 Gong, Q. H., Wang, Q., Pan, L. L., Liu, X. H., Huang, H., & Zhu, Y. Z. (2010). Hydrogen sulfide attenuates lipopolysaccharide-induced cognitive impairment: a pro-inflammatory pathway in rats. Pharmacology, biochemistry, and behavior, 96(1), 52-58. doi: 10.1016/j.pbb.2010.04.006
 Hunter, J. P., Hosgood, S. A., Patel, M., Furness, P., Sayers, R. D., & Nicholson, M. L. (2015). Hydrogen sulfide reduces inflammation following abdominal aortic occlusion in rats. Annals of vascular surgery, 29(2), 353-360. doi: 10.1016/j.avsg.2014.11.002
 Issa, K., Kimmoun, A., Collin, S., Ganster, F., Fremont-Orlowski, S., Asfar, P., … & Levy, B. (2013). Compared effects of inhibition and exogenous administration of hydrogen sulphide in ischaemia-reperfusion injury. Critical care (London, England), 17(4), R129. doi: 10.1186/cc12808
 Jackson, M. R., Melideo, S. L., & Jorns, M. S. (2012). Human sulfide:quinone oxidoreductase catalyzes the first step in hydrogen sulfide metabolism and produces a sulfane sulfur metabolite. Biochemistry, 51 (34), 6804-6815. doi: 10.1021/bi300778t
 Javed, A., Parvaiz, F., & Manzoor, S. (2019). Bacterial vaginosis: An insight into the prevalence, alternative treatments regimen and it's associated resistance patterns. Microbial pathogenesis, 127, 21-30. doi: 10.1016/j.micpath.2018.11.046
 Kenyon, C., Colebunders, R., & Crucitti, T. (2013). The global epidemiology of bacterial vaginosis: a systematic review. American journal of obstetrics and gynecology, 209(6), 505-523. doi: 10.1016/j.ajog.2013.05.006
 Lan, A., Liao, X., Mo, L., Yang, C., Yang, Z., Wang, X., … & Xiao, L. (2011). Hydrogen sulfide protects against chemical hypoxia-induced injury by inhibiting ROS-activated ERK1/2 and p38MAPK signaling pathways in PC12 cells. PloS one, 6(10), e25921. doi: 10.1371/journal.pone.0025921
 Liu, F., Liu, G. J., Liu, N., Zhang, G., Zhang, J. X., & Li, L. F. (2015). Effect of hydrogen sulfide on inflammatory cytokines in acute myocardial ischemia injury in rats. Experimental and therapeutic medicine, 9(3), 1068-1074. doi: 10.3892/etm.2015.2218
 Łowicka, E., & Bełtowski, J. (2007). Hydrogen sulfide (H2S) – the third gas of interest for pharmacologists. Pharmacological reports, 59(1), 4-24. PMID: 17377202
 McEvoy, G. K., & Snow, E. K. (Eds.). (2019). AHFS Drug Information (2019). American Society of Health-System Pharmacists.
 Nair, A. B., & Jacob, S. (2016). A simple practice guide for dose conversion between animals and human. Journal of basic and clinical pharmacy, 7(2), 27-31. doi: 10.4103/0976-0105.177703
 Paul, B. D., & Snyder, S. H. (2015). H2S: A Novel Gasotransmitter that Signals by Sulfhydration. Trends in biochemical sciences, 40(11), 687-700. doi: 10.1016/j.tibs.2015.08.007
 Reda, S., Gonçalves, F. A., Mazepa, M. M., & De Carvalho, N. S. (2018). Women infected with HIV and the impact of associated sexually transmitted infections. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 142(2), 143-147. doi: 10.1002/ijgo.12507
 Reiter, S., & Kellogg Spadt, S. (2019). Bacterial vaginosis: a primer for clinicians. Postgraduate medicine, 131 (1), 8-18. doi: 10.1080/00325481.2019.1546534
 Rinaldi, L., Gobbi, G., Pambianco, M., Micheloni, C., Mirandola, P., & Vitale, M. (2006). Hydrogen sulfide prevents apoptosis of human PMN via inhibition of p38 and caspase 3. Laboratory investigation; a journal of technical methods and pathology, 86(4), 391-397. doi: 10.1038/labinvest.3700391
 Schuh, K., & Pahl, A. (2009). Inhibition of the MAP kinase ERK protects from lipopolysaccharide-induced lung injury. Biochemical pharmacology, 77(12), 1827-1834. doi: 10.1016/j.bcp.2009.03.012
 Shibuya, N., & Kimura, H. (2013). Production of hydrogen sulfide from d-cysteine and its therapeutic potential. Front Endocrinol (Lausanne), 4, 87. doi: 10.3389/fendo.2013.00087
 Tomás, M., Palmeira-de-Oliveira, A., Simões, S., Martinez-de-Oliveira, J., & Palmeira-de-Oliveira, R. (2020). Bacterial vaginosis: Standard treatments and alternative strategies. International journal of pharmaceutics, 587, 119659. doi: 10.1016/j.ijpharm.2020.119659
 Voloshchuk, N. I., Taran, I. V., & Melnik, A. V. (2015). Vascular mechanism in the formation of diclophenac induced gastrotoxicity: the association with the level of hydrogen sulfide. Curierul medical, 58(1), 7-11.
 Xiao, Q., Ying, J., Xiang, L., & Zhang, C. (2018). The biologic effect of hydrogen sulfide and its function in various diseases. Medicine, 97(44), e13065. doi: 10.1097/MD.0000000000013065
 Zimmermann, K. K., Spassov, S. G., Strosing, K. M., Ihle, P. M., Engelstaedter, H., Hoetzel, A., & Faller, S. (2018). Hydrogen Sulfide Exerts Anti-oxidative and Anti-inflammatory Effects in Acute Lung Injury. Inflammation, 41(1), 249-259. doi: 10.1007/s10753-017-0684-4
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