Evaluation of the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats

  • D. I. Grebeniuk National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • N. I. Voloshchuk National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • I. V. Taran National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • P. P. Gormash Utility non-profit enterprise “Vinnitsa Regional Pathological Bureau of Vinnitsa Regional Council”, Vinnytsya, Ukraine
  • O. A. Nazarchuk National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Keywords: hydrogen sulfide, vaginal wall, TNF-α, IL-1β, clindamycin phosphate, rats.

Abstract

Various pathological conditions can be characterized not only by a decrease or increase in basal levels of hydrogen sulfide in the serum, but also the levels of hydrogen sulfide can modulate the course of the pathological process. The impact of serum hydrogen sulfide on the condition of the intact vaginal wall of rats was evaluated in this study. The aim of the study was to evaluate the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats. The study was performed on 75 female Wistar rats under 1 year of age and weighing 160.0 to 200.0 grams. All animals were divided into 6 groups: control (intact rats); experimental 1 (H2S excess); experimental 2 (H2S deficiency); experimental 3 (intravaginal administration of suppositories with clindamycin); experimental 4 (H2S excess + suppositories with clindamycin); experimental 5 (H2S deficiency + suppositories with clindamycin). The levels of serum hydrogen sulfide were studied, as well as microscopic examination of the structure of the vaginal wall and determination of the levels of TNF-α and IL-1β in tissue homogenate were performed. In experimental groups 3, 4 and 5 all studies were performed in dynamics – 10 minutes, 4, 8 and 24 hours after a single intravaginal administration of clindamycin phosphate. The data were processed using the statistical software package SPSS 20.0 for Windows. Under conditions of both hydrogen sulfide deficiency and excess, no statistically significant changes in TNF-α and IL-1β levels in the vaginal wall of intact rats were observed. Also, no changes in the histological structure of the wall were found. Similar data were demonstrated in experimental groups 3, 4 and 5. This picture is explained by the fact that hydrogen sulfide affects various parts of the inflammatory process, while reducing the production of inflammatory mediators. In intact tissues, in the absence of an inflammatory process, there is no point of application of hydrogen sulfide, and therefore no significant changes are observed. Thus, both excess and deficiency of serum hydrogen sulfide do not affect the condition of the vaginal wall of intact rats.

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Published
2020-12-28
How to Cite
Grebeniuk, D. I., Voloshchuk, N. I., Taran, I. V., Gormash, P. P., & Nazarchuk, O. A. (2020). Evaluation of the effect of excess and deficiency of serum hydrogen sulfide on the condition of the vaginal wall of intact rats. Biomedical and Biosocial Anthropology, (41), 24-29. https://doi.org/10.31393/bba41-2020-04

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